Left: Starch and water
Middle: Water and iodine
Right: Starch, water, and iodine.
Hypothesis: When amylase is exposed to denaturing conditions, such as extreme heat, acidic conditions or high salt concentrations, it will no longer catalyze the hydrolysis of starch to maltose.
This experiment was similar to the one we did in class. At the beginning of the experiment, the following was added to each tube:
Tube1: Starch and water
Tube 2: Starch, water and untreated amylase.
Tube 3: Starch, water, and amylase treated with HCl .
Tube 4: Starch, water, and boiled amylase .
The tubes were incubated at 37 degrees C for several minutes (to allow the amylase to react with the starch). Iodine was added to each tube to test for the presence of starch. A blue reaction indicates that starch was present. If starch was still present in the tube that contained some form of amylase after the incubation period, this means that the amylase was denatured.
The enzyme lab movie
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Written by Michelle Furlong and media by Christopher Kodani and Barbara Foster, Clayton State University Morrow, GA Copyright 2002 – Department of Natural Sciences, Clayton State University. Do not copy or use anything from these pages without written permission.